United Therapeutics Intern - Regenerative Medicine in United States
The Job Details are as follows:
The intern will complete a focused research project to address a question that is relevant to the development of a novel cell- or tissue-based product for the treatment of pulmonary hypertension and other lung diseases. The research project will be chosen based upon the background of the applicant as well as the needs of the research team. Examples of research projects include:
Determine the requirements for mucociliary differentiation of airway basal cells in an engineered lung scaffold
Evaluate the presence of reactive oxygen species in cells or tissues after hypoxic and/or cold stressors, and develop strategies to mitigate damage from reactive oxygen species
Construct mathematical models of pulmonary flow based on physiological measurements and anatomy
Determine the conditions to culture airway epithelial cells in 3-dimensional cell culture bioreactors
Optimize cell culture media formulations to maximize yield and quality of cell cultures.
Examine characteristics of engineered lung tissues to provide insight into factors that impact tissue function, such as cell coverage and distribution, tissue structure analysis, cell phenotype & protein expression, and cell cycle analysis.
Key Accountabilities / Responsibilities:
Plan and execute experiments
Follow prescribed laboratory methods and protocols
Laboratory activities may involve the following, depending on the objectives of the research project:
Perform analytical and bioanalytical assays to characterize cellular phenotypes and/or matrix composition (Western Blot, PCR, flow cytometry, ELISA, immunohistochemistry, immunofluorescence)
Conduct experiments with 3D cell bioreactors, optimizing culture conditions, enumerating growth kinetics, characterizing and assessing quality metrics using standard operating procedures
Perform mammalian (primarily human) cell culture and exhibit good aseptic technique
Isolate and characterize secreted micro vesicles derived from primary cell culture
Perform and assist with lung scaffold reseeding experiments, including performance of biological assays to evaluate cell distribution and phenotype in engineered tissues
Perform histological staining of recelled lung tissue (IF & IHC), followed by microscopy imaging and analysis of stained sections
Perform assays designed to measure vascular permeability (cell junction formation, leakiness, glycocalyx assessment)
Perform cell biological assays (ELISA, histological staining and microscopy analysis, cell culture, cell viability assessment, etc.) for analysis of vascular lung recells.
Analyze cell culture media for presence of key growth factors and metabolites (ELISA, etc.)
Generate data that addresses an insightful question, produce summary report(s) and conclusions to document the outcomes of experimental testing
Summarize and document data and research findings in laboratory notebook system
Report and discuss results with mentor
Regularly present plans and data with scientists, engineers and laboratory staff
Present data to management, team leaders, and research staff at a company-wide poster session
Intern: Rising sophomore, junior or senior in Biology, Cell and Molecular Biology, Biomedical Sciences, Biomedical Engineering, or a related discipline
Advanced Intern: Enrolled in a Master’s or PhD program in Biology, Cell and Molecular Biology, Biomedical Sciences, Biomedical Engineering, or a related discipline
Preferred Knowledge, Skills, and Abilities:
Knowledge of Microsoft Excel and PowerPoint
Understanding of GraphPad/Prism, ImageJ (FIJI), or MATLAB a plus
Experience with Python a plus
The successful candidate will be motivated, curious and hard-working.
The ability to think creatively and learn quickly will contribute to successful and timely completion of goals
United Therapeutics Corporation is an Equal Opportunity/Affirmative Action Employer - EOE Minorities / Females / Protected Veterans / Individuals with Disabilities
United Therapeutics currently has five approved products on the market, and a long-term mission of providing an unlimited supply of transplantable organs for those who need them! Our employees can be found collaborating across the United States, Europe and Asia.
We challenge our employees with innovative and revolutionary projects, offer an environment which fosters high level job performance, and provide state of the art facilities in which to flourish. This is what makes United Therapeutics a stimulating place to work. We strive to be an organization that engages the minds, hearts, and most spirited efforts of each of our employees. Our sense of purpose transforms what we do from work into mission, occupation into vocation, and achievement into success.